Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies
Patent 4939264 Issued on July 3, 1990. Estimated Expiration Date: 
July 3, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
July 3, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesAntigens and immunoassays for morphine and related 3-oxybenzomorphan compounds Receptor fluorescent immunoassay Fluorescent labeling reagents containing the fluorescein and eosin chromophores Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins Substituted carboxyfluoresceins Fluorescence polarization immunoassay utilizing substituted carboxyfluoresceins Patent #: 4668640 InventorsAssigneeApplicationNo. 885054 filed on 07/14/1986US Classes:436/537, Signal modification or steric inhibition436/543, INVOLVING PRODUCING OR TREATING ANTIGEN OR HAPTEN436/546, Fluorescent label436/547, INVOLVING PRODUCTION OR TREATMENT OF ANTIBODY436/800, FLUORESCENT DYES (E.G., RHODAMINE, ETC.)436/805, OPTICAL PROPERTY436/825, PRETREATMENT FOR REMOVAL OF INTERFERING FACTORS FROM SAMPLE436/826, ADDITIVES (E.G., BUFFERS, DILUENTS, PRESERVATIVES)530/389.8, Binds drug, hapten, hapten-carrier complex, or specifically-identified chemical structure (e.g., theophylline, digoxin, etc.)530/807, HAPTEN CONJUGATED WITH PEPTIDE OR PROTEIN546/44, One of the five cyclos is five-membered and includes ring chalcogen (e.g., codeine, morphine, etc.)546/45, Nitrogen or divalent chalcogen double bonded directly to carbocyclic ring of the pentacyclo ring system (e.g., morphinones, etc.)546/46A single chalcogen is bonded directly to ring carbon of the pentacyclo ring system (e.g., desoxy morphine, etc.)ExaminersPrimary: Warden, Robert J.Assistant: Benson, Robert Attorney, Agent or FirmInternational ClassG01N 033/542AbstractThe present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.Other References
Field of SearchHAPTEN CONJUGATED WITH PEPTIDE OR PROTEINOne of the five cyclos is five-membered and includes ring chalcogen (e.g., codeine, morphine, etc.) Nitrogen or divalent chalcogen double bonded directly to carbocyclic ring of the pentacyclo ring system (e.g., morphinones, etc.) A single chalcogen is bonded directly to ring carbon of the pentacyclo ring system (e.g., desoxy morphine, etc.) INVOLVING KINETIC MEASUREMENT OF ANTIGEN-ANTIBODY REACTION Signal modification or steric inhibition Fluorescent label INVOLVING PRODUCTION OR TREATMENT OF ANTIBODY FLUORESCENT DYES (E.G., RHODAMINE, ETC.) OPTICAL PROPERTY PRETREATMENT FOR REMOVAL OF INTERFERING FACTORS FROM SAMPLE ADDITIVES (E.G., BUFFERS, DILUENTS, PRESERVATIVES) |
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