Nucleic acid hybridization assays
May 15, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDye tagged reagent Specific DNA probes in diagnostic microbiology Detection of microbial nucleic acids by a one-step sandwich hybridization test Nucleic acid hybridization assay employing antibodies to intercalation complexes Fluoroimmunoassaying Magnetic particles for use in separations Detecting polynucleotide sequences Polymerization-induced separation assay using recognition pairs Methods and kits for performing nucleic acid hybridization assays Polynucleotide determination with selectable cleavage sites InventorsAssigneeApplicationNo. 837701 filed on 03/07/1986US Classes:435/6, Involving nucleic acid435/803, PHYSICAL RECOVERY METHODS (E.G., CHROMATOGRAPHY, GRINDING)436/501BIOSPECIFIC LIGAND BINDING ASSAYExaminersPrimary: Yarbrough, Amelia B.Attorney, Agent or FirmForeign Patent References
International ClassC12Q 001/68AbstractA method for carrying out a nucleic acid hybridization test to detect a target nucleic acid sequence, the method including the steps of(1) providing a polymer molecule bonded to a first single-stranded nucleic acid sequence which is either (a) a first probe capable of hybridizing to the target nucleic acid sequence, or (b) a second nucleic acid sequence capable of hybridizing to a third nucleic acid sequence bonded to a first probe capable of hybridizing to the target nucleic acid sequence; provided that, where the polymer molecule comprises DNA, the DNA is of heterogeneous base sequence;(2) where the polymer used in step (1) is bonded to (b), providing the first probe bonded to the third nucleic acid sequence;(3) denaturing the target nucleic acid sequence;(4) contacting the denatured target nucleic acid sequence with the polymer molecule bonded to the first single-stranded nucleic acid sequence and, if the first nucleic acid sequence is (b), to the first probe bonded to the third nucleic acid sequence; and(5) detecting complexes between the target nucleic acid sequence and the polymer molecule.Other References
|
| ||||||||||||||
