Resolution of glycidyl esters to high enantiomeric excess
August 24, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3899395 3901763 3905870 Hydrophobic noncovalent binding of proteins to support materials Method for purifying cholesterol esterase Protease-free proteins, and methods of manufacturing and using the same Method for immobilizing enzymes Immobilization of enzymes Method for enzymatic transesterification of lipid and enzyme used therein Immobilized enzyme composite/process using a mica carrier InventorsAssigneeApplicationNo. 236616 filed on 08/24/1988US Classes:435/117, Preparing heterocyclic carbon compound having only O, N, S, Se, or Te as ring hetero atoms435/123, Oxygen as only ring hetero atom435/132, Preparing oxygen-containing organic compound435/157, Acyclic435/174, CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF435/176, Enzyme or microbial cell is immobilized on or in an inorganic carrier435/177, Enzyme or microbial cell is immobilized on or in an organic carrier435/180, Carrier is synthetic polymer435/197, Carboxylic ester hydrolase (3.1.1)435/198, Triglyceride splitting (e.g., lipase, etc. (3.1.1.3))435/280Resolution of optical isomers or purification of organic compounds or composition containing sameExaminersPrimary: Lilling, Herbert J.Assistant: Zitomer, Stephanie W. Attorney, Agent or FirmForeign Patent References
International ClassesC12P 017/00C12P 017/02 C07P 041/00 C12N 009/20 AbstractA method of resolving glycidyl esters to high enantiomeric excess involves fractionation of hydrolytic enzymes (e.g. lipases) to prepare biocatalysts with high enantioselectivity, and using these catalysts to selectively hydrolyze one enantiomer of the glycidyl esters. Also a method for fractionation includes stirring an aqueous solution of the enzyme with a solid inert adsorbent to provide an adsorbed and non-adsorbed enzyme fraction, where the non-adsorbed fraction displays a higher enantioselectivity than the crude non-fractionated enzyme. Also a composition composed of a glycidyl ester is obtained with an enantiomeric excess of greater than or equal to 97%.Other References
Field of SearchResolution of optical isomers or purification of organic compounds or composition containing sameEnzyme or microbial cell is immobilized on or in an inorganic carrier Carrier is synthetic polymer Carboxylic ester hydrolase (3.1.1) Acyclic Oxygen as only ring hetero atom Triglyceride splitting (e.g., lipase, etc. (3.1.1.3)) Carbohydrate is cellulose or derivative thereof Preparing oxygen-containing organic compound CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF ENZYME SEPARATION OR PURIFICATION By sorption Enzyme or microbial cell is immobilized on or in an organic carrier |
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