Vesicles resistant to enzyme lysis and use thereof in an enzyme assay
Patent 4888288 Issued on December 19, 1989. Estimated Expiration Date: 
December 19, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
December 19, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesStructural analogs of natural phospholipids Use of phosphotriester intermediates for preparation of functionalized liposomes Synthetic phosphatidyl cholines useful in forming liposomes Spontaneous preparation of small unilamellar liposomes Patent #: 4619794 InventorApplicationNo. 824362 filed on 01/30/1986US Classes:435/21, Involving phosphatase264/4, ENCAPSULATING NORMALLY LIQUID MATERIAL424/450, Liposomes428/402.2, Microcapsule with fluid core (includes liposome)435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP435/7.9, Assay in which an enzyme present is a label435/19, Involving esterase435/177, Enzyme or microbial cell is immobilized on or in an organic carrier435/182, Enzyme or microbial cell is entrapped within the carrier (e.g., gel, hollow fibre)436/829, LIPOSOMES (E.G., ENCAPSULATION, ETC.)514/76, Amine addition salt of organic phosphorus containing acid514/77, Inner salt (e.g., betaine, etc.)514/78LecithinsExaminersPrimary: Rosen, SamAttorney, Agent or FirmInternational ClassC12Q 001/42AbstractLiposomes are formed from compounds including a phosphonate or phosphinate group instead of a phosphate group. The liposomes bind to enzymes, such as a phospholipase, and such liposomes containing a detectable marker may be used as a substrate in an enzyme immunoassay.Other References
Field of SearchLiposomesEnzyme or microbial cell is immobilized on or in an organic carrier Enzyme or microbial cell is entrapped within the carrier (e.g., gel, hollow fibre) MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP Involving esterase Involving phosphatase LIPOSOMES (E.G., ENCAPSULATION, ETC.) Inner salt (e.g., betaine, etc.) Amine addition salt of organic phosphorus containing acid Lecithins Chalcogen attached indirectly to the phosphorus by acyclic nonionic bonding Microcapsule with fluid core (includes liposome) |
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