U.S. patents available from 1976 to present.
U.S. patent applications available from 2005 to present.

Analysis of samples by electrophoresis using a charge coupled device

Patent 4874492 Issued on October 17, 1989. Estimated Expiration Date: Icon_subject June 6, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Method of measuring total protein of sample with the aid of electrophoretic image Patent #: 4666578
Issued on: 05/19/1987
Inventor: Yamamoto

Inventor

Assignee

Application

No. 203482 filed on 06/06/1988

US Classes:

204/461, With analysis or detailed detection204/612With detailed detection system (e.g., including a light source and a camera, etc.)

Examiners

Primary: Niebling, John F.
Assistant: Starsiak, John S. Jr.

Attorney, Agent or Firm

Foreign Patent References

  • 125691 EP. 11/13/1984
  • 143205 EP. 06/13/1985
  • WO87/07033 WO. 11/13/1987

International Class

G01N 027/26

Foreign Application Priority Data

1985-05-29 GB

Abstract

Light from the pattern of spots of bands resulting from electrophoresis is detected by a two-dimensional charge coupled device, visualization of the spots or band being achieved by irradiating the gel with a U/V source to stimulate fluorescent markers preferably applied before running of the gel. The charge coupled device is cooled to suppress thermal dark current and, more particularly, to improve sensitivity and dynamic range.

Other References

  • Toda et al, "Microcomputer-Aided Two-Dimensional Densitometry", Electrophoresis '83, (1984), pp. 139-146
  • Smith et al, "Fluorescence Detection in Automated DNA Sequence Analysis", Nature, vol. 321 (Jun. 12, 1986), pp. 674-679
  • Bonazzola et al, "Charged Coupled Devices as Autoradiography Imagers", IEEE Transactions on Nuclear Science, vol. NS-32, No. 1 (Feb. 1985), pp. 567-570
  • Sutherland et al, "Electronic Imaging System for Direct and Rapid Quantitation of Fluorescence from Electrophoretic Gels", Analytical Biochemistry 163 (1987), pp. 446-45
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