Reconstituting viral glycoproteins into large phospholipid vesicles
April 13, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesBiological preparations Biological preparations Viral liposome particle Method of encapsulating biologically active materials in lipid vesicles Viral liposome particle Method of preparing an immunogenic membrane protein aggregate of influenza and parainfluenza viruses and rhabdoviruses Treatment of malaria with liposomes containing 8-aminoquinoline derivatives and glycoconjugates Method for improving reformer yield selectivity Immunogenic protein or peptide complex, method or producing said complex and the use thereof as an immune stimulant and as a vaccine Patent #: 4578269 InventorsAssigneeApplicationNo. 07/037751 filed on 04/13/1987US Classes:264/4.6, Solid-walled microcapsule formed by physically removing a constituent (e.g., evaporation, extraction, etc.)264/4.3, With treatment subsequent to solid wall formation (e.g., coating, hardening, etc.)424/1.21, Molecular bilayer structure (e.g., vesicle, liposome)424/450, Liposomes428/402.2, Microcapsule with fluid core (includes liposome)436/829, LIPOSOMES (E.G., ENCAPSULATION, ETC.)514/8, Glycoprotein (carbohydrate containing)514/885IMMUNE RESPONSE AFFECTING DRUGExaminersPrimary: Lovering, Richard D.Attorney, Agent or FirmInternational ClassesA61K 9/127 (20060101)A61K 39/145 (20060101) A61K 39/155 (20060101) A61K 39/00 (20060101) AbstractThe present disclosure relates to novel liposome compositions and methods for their preparation. Utilization of the present invention provides an efficient reconstitution of membrane proteins into large (0.1 to 2 micron diameter) phospholipid vesicles with a large, internal aqueous space. The method has been exemplified with the use of glycoproteins of influenza (A/PR8/34) and Sendai (parainfluenza type I) viruses. The method comprises (A) extracting out the desired membrane protein from a source biological material with an extraction buffer comprising a detergent; (B) mixing the extract with a phospholipid solution and deriving a cochleate intermediate; and (C) forming large phospholipid vesicles with integrated membrane protein in a biologically active state.Other References
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