Solution phase nucleic acid sandwich assay

Patent 4868105 Issued on September 19, 1989. Estimated Expiration Date:

September 19, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Nucleic acid hybridization assay employing antibodies to intercalation complexes
Patent #: 4563417
Issued on: 01/07/1986
Inventor: Albarella , et al.

Detecting polynucleotide sequences
Patent #: 4716106
Issued on: 12/29/1987
Inventor: Chiswell

Arrays of alternating nucleic acid fragments for hybridization arrays
Patent #: 4731325
Issued on: 03/15/1988
Inventor: Palva , et al.

Methods and kits for performing nucleic acid hybridization assays Patent #: 4751177
Issued on: 06/14/1988
Inventor: Stabinsky

Inventors

Assignee

Chiron Corporation

Application

No. 06/807624 filed on 12/11/1985

US Classes:

435/6, Involving nucleic acid435/810, PACKAGED DEVICE OR KIT536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA

Examiners

Primary: Warden, Robert J.
Assistant: Benson, Robert

Attorney, Agent or Firm

Irell & Manella

International Classes

C12Q 1/68 (20060101)
C12Q 1/70 (20060101)
C07H 21/00 (20060101)

Abstract

Methods and compositions are provided for rapid detection of nucleic acid sequences. The method employs two reagent sets. The first set is a labelling set comprising: (1) a first nucleic acid sequence probe having an analyte complementary region and a first recognition sequence region and (2) a labelled sequence complementary to the first recognition sequence region. The second set is a capturing set comprising: (1) a second nucleic acid sequence probe having an analyte complementary region and a second recognition sequence region, (2) a specific binding pair member conjugated to a sequence complementary to the second recognition sequence, and (3) a separating means to which is bound a complementary specific binding pair member. The sample and probes are combined under annealing conditions, followed by addition of the other reagents, separation of the bound label from the supernatant and detection of the label in either phase.

Other References

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