Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups

Patent 4849513 Issued on July 18, 1989. Estimated Expiration Date:

July 18, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Phosphoramidite nucleoside compounds Patent #: 4668777
Issued on: 05/26/1987
Inventor: Caruthers , et al.

Inventors

Assignee

California Institute of Technology

Application

No. 06/878045 filed on 06/24/1986

US Classes:

536/26.6, Labelled (e.g., tagged with radioactive tracer, fluorescent marker, intercalator, etc.)536/25.32, Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.)536/25.34, Trivalent phosphorus compound utilized536/26.8, The N-hereto ring is six-membered and monocyclic (e.g., uridine-5`-mono- phosphate, etc.)536/28.53, Chalcogen bonded directly to the 2- and 4-positions of the diazine ring (e.g., uridine, etc.)536/28.54Alkyl, or substituted alkyl, bonded directly to the 5-position of the diazine ring (e.g., thymidine, 5-methyl uridine, etc.)

Examiners

Primary: Brown, Johnnie R.
Assistant: Tou, Jenny

Attorney, Agent or Firm

Mueth; Joseph E.

International Classes

C12Q 1/68 (20060101)
C07H 21/00 (20060101)

Abstract

The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.