Signal amplification in an assay employing a piezoelectric oscillator
Patent 4847193 Issued on July 11, 1989. Estimated Expiration Date: 
June 18, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
June 18, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod for the assay of classes of antigen-specific antibodies Immunoassay for antigens Sandwich immunoassay using piezoelectric oscillator Magnetic particles for use in separations Sensor having piezoelectric crystal for microgravimetric immunoassays Patent #: 4735906 InventorsAssigneeApplicationNo. 07/064266 filed on 06/18/1987US Classes:435/6, Involving nucleic acid436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/524, Carrier is inorganic436/525, Metal or metal coated436/528, Carrier is organic436/531, Carrier is synthetic resin436/806ELECTRICAL PROPERTY OR MAGNETIC PROPERTYExaminersPrimary: Warden, Robert J.Assistant: Wieder, Stephen C. International ClassesC12Q 1/68 (20060101)G01N 27/00 (20060101) G01N 33/543 (20060101) AbstractA method for detecting a first member of a specific binding pair in a sample, the first member being capable of forming a complex with a second member of the specific binding pair, includes immobilizing the second member on a surface of a piezoelectric oscillator, determining a first vibrational frequency of the oscillator on which the second member has been immobilized, contacting the surface of the oscillator on which the second member has been immobilized with the sample under conditions permitting formation of the complex, and with a particle under conditions permitting binding of the particle with the first member, mass coupling the bound particle with the oscillator, and determining a second vibrational frequency of the oscillator with which the particle has been mass coupled, the difference between the first vibrational frequency and the second vibrational frequency providing a measure of the quantity of the first member in the sample.Other References
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