Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays
Method for electroblotting macromolecules from a chromatographic gel
Method for electroblotting
Device for horizontal electroblotting Patent #: 4622124
ApplicationNo. 07/049739 filed on 05/13/1987
US Classes:204/464, Blotting204/614Blotter (e.g., membrane, etc.)
ExaminersPrimary: Niebling, John F.
Assistant: Starsiak, John S. Jr.
Attorney, Agent or Firm
International ClassG01N 27/447 (20060101)
AbstractAn apparatus especially suitable for transferring a particular specimen from a polyacrylamide electrophoresis or like gel onto a support sheet by means of electroblotting is disclosed herein along with its method of operation. This apparatus utilizes a pair of plate-shaped electrode assemblies, each of which in the disclosed embodiment is a multilayered assembly including an electrode layer of electrically conductive material and a layer of thermally conductive material which serves as a heat sink. These electrode assemblies are adjustably supported in spaced-apart but confronting relationship to one another with the gel and support sheet sandwiched therebetween. At the same time, the electrode layers are connected to a source of power for producing an electric field across the gel and support sheet in order to transfer the specimen from the gel to the sheet by means of electroblotting. In the specific embodiment disclosed, the spacing between the electrode assemblies can be adjustably decreased at a number of different points in order to ensure that they remain parallel to one another in their adjusted positions whereby to squeeze the gel and support sheet uniformly along the extend of the gel.