Process for the rapid and simple isolation of nucleic acids
Patent 4830969 Issued on May 16, 1989. Estimated Expiration Date: 
May 16, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
May 16, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References Re25785 3585179 3681195 Method for the genetic detection of microorganisms Process for the production of microorganism lysates Preparation of soluble edible protein from leafy green crops Manufacture of yeast protein isolate having a reduced nucleic acid content by a thermal process Test kit for the genetic detection of microorganisms Preparation of food functional proteins Co-integrate plasmids and their construction from plasmids of Escherichia and Streptomyces Patent #: 4338400 InventorAssigneeApplicationNo. 06/298064 filed on 08/31/1981US Classes:435/259, Lysis of micro-organism426/60, Of or with yeast or mold435/264, Cleaning using a micro-organism or enzyme435/267, Treating animal or plant material or micro-organism435/270, Removing nucleic acid from intact or disrupted cell435/272, Proteinaceous material recovered or purified435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/820, SUBCELLULAR PARTS OF MICRO-ORGANISMS435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/91.32, Prepared from virus, prokaryotic acid435/91.33, Involving virus435/91.4, Modification or preparation of a recombinant DNA vector530/344, Separation or purification530/412, Separation or purification530/417, Chromatography or by septum selective as to material, e.g., gel filtration, molecular sieve dialysis, etc.530/419, With added material530/423, Oxygenated material530/820, PROTEINS FROM MICRO-ORGANISMS536/25.4, Separation or purification of polynucleotides or oligonucleotides536/25.41Extraction processes (e.g., solvent extraction process, etc.)ExaminersPrimary: Kepplinger, Esther M.Attorney, Agent or FirmInternational ClassesC12P 19/00 (20060101)C12P 19/34 (20060101) C12N 15/10 (20060101) AbstractA process for the separation from other cellular materials of heat agglomeration resistant water soluble nitrogen containing organic compounds such as plasmids, RNA's, mitochondrial DNA's, viral DNA's, chloroplast DNA's, other episomal DNA's and certain proteins. The process comprises heating cellular materials in a solution of lysing agent to lyse the desired cells and to agglomerate water soluble nitrogen containing compounds such as certain chromosomal DNA's which are not resistant to agglomeration; centrifuging the resulting product to remove water soluble agglomerated materials; separating the supernatant liquid and precipitating the water soluble agglomeration resistant organic compounds with a water soluble precipitant. The process also includes separating the agglomeration resistant water soluble nitrogen containing compounds from each other by means of exclusion chromotography.Other References
Field of SearchProduced by the action of a carbohydrase (e.g., maltose by the action of alpha amylase on starch, etc.)Treating animal or plant material or micro-organism PROCESS OF UTILIZING AN ENZYME OR MICRO-ORGANISM TO DESTROY HAZARDOUS OR TOXIC WASTE, LIBERATE, SEPARATE, OR PURIFY A PREEXISTING COMPOUND OR COMPOSITION THEREFORE; CLEANING OBJECTS OR TEXTILES Lysis of micro-organism Removing nucleic acid from intact or disrupted cell Proteinaceous material recovered or purified SUBCELLULAR PARTS OF MICRO-ORGANISMS |
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