Specific binding assay method and reagent means
Nucleic acid hybridization assay employing antibodies to intercalation complexes Patent #: 4563417
ApplicationNo. 06/811034 filed on 12/18/1985
US Classes:435/6, Involving nucleic acid435/810, PACKAGED DEVICE OR KIT436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA
ExaminersPrimary: Warden, Robert J.
Assistant: Spiegel, Jack
Attorney, Agent or Firm
International ClassC12Q 1/68 (20060101)
AbstractA diagnostic reagent is disclosed which is capable of binding to a target nucleotide sequence which is bound to a labeled polynucleotide in a target binding region which is at least partially co-extensive with the target binding region in the probe polynucleotide which is capable of binding to the target nucleotide sequence. A method is disclosed in which the reagent is contacted with a sample and with a capturing polynucleotide under conditions such that target nucleotide which may be present in the sample binds to the probe polynucleotide and displaces labeled polynucleotide from the reagent complex, and the capturing polynucleotide binds selectively to the displaced labeled polynucleotide in the region of the labeled polynucleotide that had been bound to the probe polynucleotide. Determination of the displaced nucleotide gives a value which is a function of the presence and concentration of target nucleotide in the sample.
Field of SearchInvolving nucleic acid