Protein immobilization by adsorption of a hydrophobic amidine protein derivative to a hydrophobic surface
Patent 4808530 Issued on February 28, 1989. Estimated Expiration Date: 
September 5, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
September 5, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3830699 Carrier-bound protein prepared by reacting the protein with an acylating or alkylating compound having a carrier-bonding group and reacting the product with a carrier Hydrophobic noncovalent binding of proteins to support materials Enzyme preparation having affinity for water-immiscible liquids Patent #: 4267273 InventorsAssigneeApplicationNo. 06/904160 filed on 09/05/1986US Classes:435/180, Carrier is synthetic polymer435/177, Enzyme or microbial cell is immobilized on or in an organic carrier530/815Carrier is a synthetic polymerExaminersPrimary: Naff, David M.Attorney, Agent or FirmInternational ClassesC07C 257/04 (20060101)C07C 257/00 (20060101) C12N 11/00 (20060101) C12N 11/08 (20060101) AbstractImmobilization of biologically active protein is carried out by reacting the protein with an imidoester or imidothioester having a hydrophobic moiety to form a hydrophobic amidine derivative of the protein, and then adsorbing the protein derivative to a hydrophobic surface. The protein can be an enzyme such as trypsin and the hydrphobic surface can be a synthetic organic polymer.Other References
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