Fused liposome and acid induced method for liposome fusion

Patent 4789633 Issued on December 6, 1988. Estimated Expiration Date:

December 6, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Method of encapsulating biologically active materials in lipid vesicles
Patent #: 4235871
Issued on: 11/25/1980
Inventor: Papahadjopoulos , et al.

Method of encapsulating biologically active materials in lipid vesicles
Patent #: 4241046
Issued on: 12/23/1980
Inventor: Papahadjopoulos , et al.

Method of inserting DNA into living cells Patent #: 4394448
Issued on: 07/19/1983
Inventor: Szoka, Jr. , et al.

Inventors

Assignee

University of Tennessee Research Corporation

Application

No. 06/602177 filed on 04/19/1984

US Classes:

435/458, The polynucleotide is coated with or encapsulated within a lipid containing material (e.g., liposome, etc.)264/4.1, Liquid encapsulation utilizing an emulsion or dispersion to form a solid-walled microcapsule (includes liposome)264/4.3, With treatment subsequent to solid wall formation (e.g., coating, hardening, etc.)264/4.6, Solid-walled microcapsule formed by physically removing a constituent (e.g., evaporation, extraction, etc.)424/417, Coated particulate form (e.g., liposome, etc.)424/420, Waxes, fatty acids, etc. (e.g., liposome, etc.)424/450, Liposomes428/402.2, Microcapsule with fluid core (includes liposome)435/243, MICRO-ORGANISM, PER SE (E.G., PROTOZOA, ETC.); COMPOSITIONS THEREOF; PROCES OF PROPAGATING, MAINTAINING OR PRESERVING MICRO-ORGANISMS OR COMPOSITIONS THEREOF; PROCESS OF PREPARING OR ISOLATING A COMPOSITION CONTAINING A MICRO-ORGANISM; CULTURE MEDIA THEREFOR435/252.1, Bacteria or actinomycetales; media therefor435/254.1, Fungi435/257.1, Algae, media therefor435/468, Introduction of a polynucleotide molecule into or rearrangement of a nucleic acid within a plant cell435/471Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within a microorganism (e.g., bacteria, protozoa, bacteriophage, etc.)

Examiners

Primary: Wiseman, Thomas G.
Assistant: Teskin, Robin L.

Attorney, Agent or Firm

International Classes

A61K 9/127 (20060101)
C12N 15/87 (20060101)
C12N 15/88 (20060101)

Abstract

Liposomes containing phosphatidylethanolamine, palmitoyl homocysteine or oleic acid or palmitic acid, fuse rapidly when the pH of the medium is reduced below 7. Liposome fusion was measured by (a) mixing of the liposomal lipids as shown by resonance energy transfer, (b) gel filtration and (c) electron microscopy. The presence of phosphatidylethanolamine or acid addition esters thereof in the liposomes greatly enhances fusion; whereas the presence of phosphatidylcholine inhibits fusion. During fusion of liposomes containing phosphatidylethanolamine:palmitoyl homocysteine (8:2), almost all of the encapsulate calcein is released. Inclusion of cholesterol (40%) in the liposomes substantially decreases leakage without impairing fusion. Those pH sensitive liposomes are fused to deliver biologically active molecules such as DNA, into living cells.

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