Autocatalytic replication of recombinant RNA
Patent 4786600 Issued on November 22, 1988. Estimated Expiration Date: 
November 22, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
November 22, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.InventorsApplicationNo. 06/614350 filed on 05/25/1984US Classes:435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/6, Involving nucleic acid435/91.3, Polynucleotide contains only ribonucleotide monomers536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/25.1, 3`-5` linked RNA536/25.32Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.)ExaminersPrimary: Wiseman, Thomas G.Assistant: Seidman, S. Attorney, Agent or FirmInternational ClassesC12P 19/00 (20060101)C12P 19/34 (20060101) C12Q 1/68 (20060101) C12N 15/10 (20060101) AbstractThis invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis and a heterologous sequence of interest inserted at a specific site in the internal region of the recombinant molecule. Such recombinant RNA molecules are capable of serving as a template for the synthesis of complementary single-stranded molecules by RNA-directed RNA polymerase. The product molecules so formed are also capable of serving as a template for the synthesis of additional copies of the original recombinant RNA molecule. In a preferred embodiment of the invention Qଲ replicase is used as the RNA-directed RNA polymerase.Other References
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