Growth of hepatitus A virus in vero cells

Patent 4783407 Issued on November 8, 1988. Estimated Expiration Date:

November 8, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Hepatitis a virus cell culture in vitro
Patent #: 4164566
Issued on: 08/14/1979
Inventor: Provost , et al.

High titer production of hepatitis A virus Patent #: 4301249
Issued on: 11/17/1981
Inventor: Markus , et al.

Inventors

Assignee

Merck & Co., Inc.

Application

No. 06/781830 filed on 09/30/1985

US Classes:

435/235.1, VIRUS OR BACTERIOPHAGE, EXCEPT FOR VIRAL VECTOR OR BACTERIOPHAGE VECTOR; COMPOSITION THEREOF; PREPARATION OR PURIFICATION THEREOF; PRODUCTION OF VIRAL SUBUNITS; MEDIA FOR PROPAGATING424/226.1Hepatitis A virus

Examiners

Primary: Kepplinger, Esther M.

Attorney, Agent or Firm

International Classes

A61K 39/29 (20060101)
C12N 7/08 (20060101)
C12N 7/04 (20060101)

Abstract

Hepatitus A virus is grown in Vero cells after passage in in vitro cell culture. Virus replication and continued passage in Vero cells requires extended incubation times, up to about four weeks, for early passages and incubation temperatures no higher than about 32° C. Continued passage results in a significant decrease in incubation time and an increase in virus yield. Cultivation of hepatitus A virus in Vero cells meets the development requirements for an inactivated human vaccine.

Other References

Kojima et al., J. of Med. Virol., 7:273-286 (1981)
Locarini et al., J. of Virol., 37:216-225 (1981)
Provost, In Vitro Propagation of Hepatitus A Virus, in Gerety (ed.) Hepatitus A, Academic Press (1984)