Unitized solid phase immunoassay kit and method
Glass support coated with synthetic polymer for bioprocess
Immunoassay with solid phase having coating containing blood platelet substitute
Immunoassay with article having support film and immunological counterpart of analyte
Polycycloalkylidene-polycycloalkanes, epidioxy derivatives thereof and method of preparation Patent #: 4705847
ApplicationNo. 06/682373 filed on 12/17/1984
US Classes:436/531, Carrier is synthetic resin427/2.13, Layer formed contains chemical reagent or chemically reacts with substrate (e.g., cell stain or fix, pH paper, immobilized antigen)435/180, Carrier is synthetic polymer435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)435/7.94, Sandwich assay436/534, Carrier is water suspendible particles436/823, IMMUNOGENIC CARRIER OR CARRIER PER SE436/824IMMUNOLOGICAL SEPARATION TECHNIQUES
ExaminersPrimary: Nucker, Christine M.
Attorney, Agent or Firm
International ClassesG01N 33/544 (20060101)
G01N 33/545 (20060101)
AbstractA solid phase immunoassay comprises the steps of(a) immobilizing an immunoreagent on the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyimides and polyfluorinated synthetic resins,(b) contacting the immunoreagent with a complementary immunoreagent whereby an immunocomplex immobilized on said carrier is formed,(c) quantitating the immobilized immunocomplex.An element useful in conducting this solid phase immunoassay is prepared by a process of treating the surface of an article comprised of a synthetic polymer selected from the group consisting of polyimides and polyfluorinated synthetic resins to make it adsorptive of an immunoreagent which comprises the steps of(a) thoroughly rinsing the surface with a water-miscible organic solvent,(b) thoroughly rinsing the surface with water.