Detection of specific DNA sequences by flow cytometry
Patent 4770992 Issued on September 13, 1988. Estimated Expiration Date: 
November 27, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
November 27, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesFlow cytometric measurement of total DNA and incorporated halodeoxyuridine Patent #: 4585736 InventorsApplicationNo. 06/802802 filed on 11/27/1985US Classes:435/6, Involving nucleic acid436/800, FLUORESCENT DYES (E.G., RHODAMINE, ETC.)436/805, OPTICAL PROPERTY536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNAExaminersPrimary: Warden, Robert J.Assistant: Benson, Robert Attorney, Agent or FirmInternational ClassesC12Q 1/68 (20060101)G01N 15/14 (20060101) AbstractA method for detection of DNA sequences. In the method, chromatin (comprising protein and DNA) is contacted with a cross-linking agent for the protein of the chromatin to provide a substantially rigid chromatin particle. The DNA of the chromatin particle is then subjected to treatment to cause a separation of the individual DNA strands into single stranded DNA. Preselected sequences of the single stranded DNA are then contacted with a complementary polynucleotide probe specific for the DNA sequence of interest. The polynucleotide probe is marked with a fluorescent label thereby labelling a target sequence of DNA in the chromatin particles. The fluorescently tagged DNA sequences are then detected by subjecting the polynucleotide probe to a suitable light source and detecting the light emitted by the fluorescent label so as to identify the preselected DNA sequence.Other References
|
| ||||||||||||||
