Cell size measurements using light in flow cytometry and cell sorting
Patent 4765737 Issued on August 23, 1988. Estimated Expiration Date: 
March 30, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
March 30, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesApparatus for counting and classifying particles Patent #: 4110043 InventorsAssigneeApplicationNo. 07/031790 filed on 03/30/1987US Classes:356/336, By particle light scattering356/73, PLURAL TEST377/11Including particle size determination variationsExaminersPrimary: Rosenberger, R. A.Attorney, Agent or FirmInternational ClassesG01N 15/14 (20060101)G01N 15/02 (20060101) G01N 15/00 (20060101) AbstractIn a flow cytometer where the laser beam is larger than the size of each biological cell of a column of cells being passed through the laser beam at a high rate wherein new and improved method and means are used to calculate the size of the cells as sensed by low angle forward light scatter or secondary emission of fluorescent material on the surface of the cell by measuring the time-of-flight of the smaller cell across the laser beam, with the time-of-flight being measured by integrating an electrical signal from either the forward light scatter or the secondary flourescent emission from a single cell as it passes through the laser beam and deriving still another electrical signal measuring the peak amplitude of the first signal and then dividing the integrated signal by the peak signal and subtracting a constant electrical signal from that quotient. | |
