ApplicationNo. 06/615966 filed on 05/31/1984
US Classes:435/34, Determining presence or kind of micro-organism; use of selective media356/36, WITH SAMPLE PREPARATION435/39, Quantitative determination435/40.51, Involving a monolayer, smear or suspension of microorganisms or cells436/10, Particle count or volume standard or control (e.g., platelet count standards, etc.)436/17, Preparation composition (e.g., lysing or precipitation, etc.)436/63BIOLOGICAL CELLULAR MATERIAL TESTED
ExaminersPrimary: Warden, Robert J.
Assistant: Deck, Randall E.
Attorney, Agent or Firm
International ClassesG01N 33/50 (20060101)
G01N 33/48 (20060101)
AbstractThis invention describes a reagent system, including saponin in a lysing reagent and a rapidly active cross-linking agent such as glutaraldehyde as a fixing reagent, which reproducably affects whole blood to cause the red blood cells to stromatolyze and modifies the leukocytes to generate data to define four distinct clusters for detection and classification by flow analysis instrumentation. The clusters represent the four major leukocyte types found in blood: lymphocytes, monocytes, neutrophils and eosinophils, thus providing a method of leukocyte differential analysis. The parameters used for the leukocyte classification include combinations of two or more of DC (Coulter) volume, high frequency (RF) size, opacity (RF size/DC volume), light scatter at various angular ranges, and fluorescence at various wavelengths of illumination.