Methods and kits for performing nucleic acid hybridization assays
Patent 4751177 Issued on June 14, 1988. Estimated Expiration Date: 
June 14, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
June 14, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesSpecific DNA probes in diagnostic microbiology Phosphoramidite compounds and processes Detection of microbial nucleic acids by a one-step sandwich hybridization test Soluble assay Patent #: 4522922 InventorAssigneeApplicationNo. 06/744509 filed on 06/13/1985US Classes:435/6, Involving nucleic acid435/810, PACKAGED DEVICE OR KIT435/814, ENZYME SEPARATION OR PURIFICATION436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/527, Glass or silica436/531, Carrier is synthetic resin436/808, Automated or kit436/824, IMMUNOLOGICAL SEPARATION TECHNIQUES536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA930/224Herpes relatedExaminersPrimary: Marantz, SidneyAssistant: Wagner, Richard Attorney, Agent or FirmInternational ClassesC12Q 1/68 (20060101)C12Q 1/70 (20060101) AbstractA method for the isolation and quantitative detection of a selected single-stranded target polynucleotide from solution. The target polynucleotide is hybridized in solution to a single-stranded mediator polynucleotide, a probe polynucleotide, and an immobilized polynucleotide sequence. The sequence of the mediator polynucleotide comprises a first sequence complementary to a first portion of the target polynucleotide sequence and a second nucleotide sequence complementary to a portion of a single-stranded immobilized polynucleotide sequence. The probe polynucleotide, which carries a detectable label, is complementary to a second portion of the necleotide sequence of the target. The immobilized polynucleotide is immobilized by attachment to a solid support, and, through hybridization to the mediator polynucleotide, functions to immobilize the entire immobilized polynucleotide/target polynucleotide/probe polynucleotide "sandwich".Other References
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