Method for producing a recombinant baculovirus expression vector
Patent 4745051 Issued on May 17, 1988. Estimated Expiration Date: May 17, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/69.51, Interferons435/243, MICRO-ORGANISM, PER SE (E.G., PROTOZOA, ETC.); COMPOSITIONS THEREOF; PROCES OF PROPAGATING, MAINTAINING OR PRESERVING MICRO-ORGANISMS OR COMPOSITIONS THEREOF; PROCESS OF PREPARING OR ISOLATING A COMPOSITION CONTAINING A MICRO-ORGANISM; CULTURE MEDIA THEREFOR435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/456, The polynucleotide is encapsidated within a virus or viral coat435/463, Involving general or homologous recombination (e.g., gene targeting, etc.)435/466, The polynucleotide is a shuttle vector or a transiently replicating hybrid vector435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/91.41, By insertion or addition of one or more nucleotides536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)930/220Viral peptide or viral protein (See note 6)
A method for producing a recombinant baculovirus expression vector, capable of expression of a selected gene in a host insect cell, is disclosed. The preferred method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant shuttle vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant shuttle vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome.
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May 17, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
