Plant cell microinjection technique

Patent 4743548 Issued on May 10, 1988. Estimated Expiration Date:

August 1, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Inventors

Assignee

Calgene, Inc.

Application

No. 06/761735 filed on 08/01/1985

US Classes:

800/278, METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART435/470Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection

Examiners

Primary: Warren, Charles F.
Assistant: Fox, David T.

Attorney, Agent or Firm

Rowland; Bertram I.

International Class

C12N 15/62 (20060101)

Abstract

Microinjection techniques for plant protoplasts utilize a holding pipette for immobilizing the protoplast while an injection pipette is utilized to inject the macromolecule. In order to manipulate the protoplasts without damage, the protoplasts are cultured for from about 1 to 5 days before the injection is performed to allow for partial regeneration of the cell wall. It was found that injection through the partially regenerated cell wall could still be accomplished and particular compartments of the cell could be targeted. The methods are particularly useful for transformation of plant protoplasts with exogenous genes.

Other References

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