Pulsed-field gradient gel electrophoretic apparatus
Patent 4740283 Issued on April 26, 1988. Estimated Expiration Date: 
February 3, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
February 3, 2007. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3506554 Method of electrophoretic purification of enzymes and peptides by means of an adjustable, specialized, geometrically located electrode system Electrophoresis using alternating transverse electric fields Method for electroblotting macromolecules from a chromatographic gel Cellular spin resonance spectrometer Patent #: 4569741 InventorsAssigneeApplicationNo. 07/005278 filed on 02/03/1987US Classes:204/458, Plural rapid changes in direction of electric field (at least 1,000 times total and at more than 1/sec) (e.g., pulsed field, etc.)204/609Plural rapid changes in direction of electric field (at least 1,000 times total and at more than 1/sec) (e.g., pulsed field, etc.)ExaminersPrimary: Niebling, John F.Assistant: Starsiak, John S. Jr. Attorney, Agent or FirmInternational ClassesB01D 57/02 (20060101)G01N 27/447 (20060101) AbstractA pulsed-field gradient gel electrophoretic apparatus is described wherein a free standing gel is employed. Electrode arrays are oriented to provide three dimensional fields across the face of the gel rather than in the plane of the gel. The electrodes produce three dimensional fields which are orthogonally oriented with respect to each other and cause the molecules to proceed down the lanes in the gel in a generally saw-tooth manner oscillating between the surfaces thereof. Alternately, three dimensional, electric fields are arranged at angles greater than 90° to one another across the face of the gel in separating the molecules, following which transfer electrodes are substituted for the separation electrodes in order to transfer the molecules from their separation lanes onto a separate substrate.Other References
|
| ||||||||||||||
