Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
Patent 4727028 Issued on February 23, 1988. Estimated Expiration Date: 
February 23, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
February 23, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesRecombinant DNA process utilizing a papilloma virus DNA as a vector Patent #: 4419446 InventorsAssigneeApplicationNo. 06/538051 filed on 09/30/1983US Classes:435/356, L cell or derivative (e.g., Ltk(-), etc.)435/194, Transferring phosphorus containing group (e.g., kineases, etc.(2.7))435/243, MICRO-ORGANISM, PER SE (E.G., PROTOZOA, ETC.); COMPOSITIONS THEREOF; PROCES OF PROPAGATING, MAINTAINING OR PRESERVING MICRO-ORGANISMS OR COMPOSITIONS THEREOF; PROCESS OF PREPARING OR ISOLATING A COMPOSITION CONTAINING A MICRO-ORGANISM; CULTURE MEDIA THEREFOR435/252.31, Bacillus (e.g., B. subtilis, B. thuringiensis, etc.)435/252.33, Escherichia (e.g., E. coli, etc.)435/252.35, Streptomyces435/254.11, Transformants435/317.1, MISCELLANEOUS (E.G., SUBCELLULAR PARTS OF MICRO-ORGANISMS, ETC.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/91.41, By insertion or addition of one or more nucleotides536/23.2, Encodes an enzyme536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)930/240Enzyme or isoenzymeExaminersPrimary: Martinell, JamesAttorney, Agent or FirmInternational ClassC12N 15/00 (20060101)AbstractThe present invention comprises novel recombinant DNA cloning and expression vectors which confer hygromycin B and/or G418 resistance to eukaryotic and prokaryotic host cells. The novel recombinant DNA vectors are derived from plasmid pKC203, a plasmid which can be isolated from E. coli JR225 (ATCC 31912). The hygromycin B and G418 resistance-conferring genes can be isolated on the 7.5 kb BglII restriction fragment or the 2.5 kb SalI-BglII restriction fragment of plasmid pKC203. The eukaryotic recombinant DNA vectors of the present invention are prepared by inserting such resistance-conferring restriction fragments into a vector, such as plasmid pSV5gpt, that comprises a eukaryotic promoter and the necessary functions for maintenance of the vector as an extrachromosomal element or as an integrated sequence in the host cell chromosomal DNA. Furthermore, the present invention comprises useful derivatives of plasmid pKC203 which, although comprising no eukaryotic elements, are useful recombinant vectors for E. coli and other prokaryotes and serve as starting material for the construction of eukaryotic vectors that confer hygromycin B and/or G418 resistance to eukaryotic host cells. One useful derivative of plasmid pKC203 is constructed by circularizing the ~7.5 kb BglIII restriction fragment of plasmid pKC203 to form plasmid pSC701, which can be further digested with HaeII or SauIIIA1 to form smaller plasmids. The present invention also comprises the novel transformants of the aforementioned recombinant DNA vectors.Other References
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