Reagent polynucleotide complex with multiple target binding regions, and kit and methods
Patent 4725536 Issued on February 16, 1988. Estimated Expiration Date: 
September 19, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
September 19, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesNucleic acid hybridization assay employing antibodies to intercalation complexes Patent #: 4563417 InventorsAssigneeApplicationNo. 06/777796 filed on 09/19/1985US Classes:435/6, Involving nucleic acid435/810, PACKAGED DEVICE OR KIT436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNAExaminersPrimary: Warden, Robert J.Assistant: Spiegel, Jack Attorney, Agent or FirmInternational ClassC12Q 1/68 (20060101)AbstractReagent complexes containing a probe polynucleotide with at least two target binding regions. A labeled polynucleotide is bound to at least a portion of each target binding region. In one method, sample nucleic acid displaces labeled polynucleotide from one target binding region; after washing, a reagent polynucleotide displaces labeled polynucleotide from the other target binding region. In a second method, sample nucleic acid strands having two target nucleic acid sequences in proximity (e.g., sequences translocated in certain cancers) displace the labeled polynucleotide from both target binding region.Field of SearchInvolving nucleic acid |
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