U.S. patents available from 1976 to present.
U.S. patent applications available from 2005 to present.

Detecting polynucleotide sequences

Patent 4716106 Issued on December 29, 1987. Estimated Expiration Date: Icon_subject February 28, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventor

Assignee

Application

No. 06/706747 filed on 02/28/1985

US Classes:

435/6, Involving nucleic acid435/5, Involving virus or bacteriophage436/501BIOSPECIFIC LIGAND BINDING ASSAY

Examiners

Primary: Warden, Robert J.
Assistant: Spiegel, Jack

Attorney, Agent or Firm

International Class

C12Q 1/68 (20060101)

Foreign Application Priority Data

1984-03-01 GB

Abstract

A method of detecting a target polynucleotide sequence in a sample uses a labelled polynucleotide secondary probe, and a polynucleotide primary probe having sequences complementary to both the target and the secondary probe. The sample, immobilized or in solution is hybridized with the primary probe; and the labelled secondary probe is also hybridized with the primary probe. The method permits the production of a labelled secondary probe which can be used in conjuction with many different primary probes for different hybridization reactions.

Other References

  • Dunn, A. R. et al., Methods in Enzymology, 65: 468-478, (1980)
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