Method of staining and identifying cells and compositions thereof
Patent 4714606 Issued on December 22, 1987. Estimated Expiration Date: 
August 13, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
August 13, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3578386 Metachromatic dye sorption means for differential determination of leukocytes Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes Individual leukocyte determination by means of differential metachromatic dye sorption Patent #: 4581223 InventorAssigneeApplicationNo. 06/765348 filed on 08/13/1985US Classes:435/40.51, Involving a monolayer, smear or suspension of microorganisms or cells435/29, Involving viable micro-organism435/34, Determining presence or kind of micro-organism; use of selective media435/39, Quantitative determination534/611The hetero ring is one of the cyclos in a polycyclo ring systemExaminersPrimary: Warden, Robert J.Assistant: Wieder, Stephen C. Attorney, Agent or FirmInternational ClassesC12Q 1/04 (20060101)G01N 1/30 (20060101) AbstractThis invention is directed to the cytology of blood, bone marrow and lymph node cells and the method of differentiating, identifying and enumerating said cells among a plurality of cells of hematopoietic origin. More specifically, the invention is directed to the use of a single cationic sulfur-containing azo dye in solution capable of staining said cells in a fixative. The dye is a water soluble quaternary azo dye identified in the Color Index as Basic Blue 41 capable of staining a plurality of cells including malignant cells of hematopoietic origin wherein the stained cells acquire individual color characteristics permitting the differentiation, identification and enumeration of each stained cell. The stained cells obtained by the process of this invention have individual color characteristics which permit the identification and differentiation by use of various instruments including, for example, an image analyzer, microscope, photomicroscope, and the like.Other References
Field of SearchBIOLOGICAL CELLULAR MATERIAL TESTED |
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