Junction-fragment DNA probes and probe clusters

Patent 4710465 Issued on December 1, 1987. Estimated Expiration Date:

December 1, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Method for cloning genes
Patent #: 4394443
Issued on: 07/19/1983
Inventor: Weissman , et al.

Method for the direct analysis of sickle cell anemia
Patent #: 4395486
Issued on: 07/26/1983
Inventor: Wilson , et al.

Method of inserting unique DNA sequences into DNA vectors
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Issued on: 07/09/1985
Inventor: Pieczenik

Recombinant DNA molecules and their use in producing human interferon-like polypeptides Patent #: 4530901
Issued on: 07/23/1985
Inventor: Weissmann

Inventors

Application

No. 06/601916 filed on 04/19/1984

US Classes:

435/6, Involving nucleic acid435/472, The polynucleotide is encapsidated within a bacteriophage, bacteriophage coat, or transducing particle435/5, Involving virus or bacteriophage435/91.53, Involving a hydrolase (3.)436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/504, Radioactive label436/811, TEST FOR NAMED DISEASE, BODY CONDITION OR ORGAN FUNCTION536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/25.32Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.)

Examiners

Primary: Warren, Charles F.
Assistant: Moskowitz, Margaret

Attorney, Agent or Firm

Ciotti, Murashige, Irell & Manella

International Class

C12Q 1/68 (20060101)

Abstract

A junction-fragment DNA probe, a DNA probe cluster, and methods of preparing and using the probe and cluster to study gene localization and organization. The probe includes first and second gene segments which are derived from first and second, single-copy genomic-DNA gene regions, respectively, separated from one another, in the genomic DNA strand, by a selected distance of between about 20 and 2,000 kilobases. The two segments in the probe are connected at a jThe invention was supported in part by the National Institute of Health grant #NIH CA-30938 and the Government has certain rights to the invention.

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