Nucleic acid hybridization assay and detectable molecules useful in such assay
Patent 4707440 Issued on November 17, 1987. Estimated Expiration Date: 
November 17, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
November 17, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesFluorescence spectroscopy assay means with fluorescent chelate of a lanthanide Detection of microbial nucleic acids by a one-step sandwich hybridization test Patent #: 4563419 InventorAssigneeApplicationNo. 06/575396 filed on 01/30/1984US Classes:435/6, Involving nucleic acid536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/25.3, Synthesis of polynucleotides or oligonucleotides536/26.14, The N-hetero ring is a diazine or a diazole ring, including hydrogenated536/26.71Preparing purine nucleotidesExaminersPrimary: Nucker, Christine M.Attorney, Agent or FirmInternational ClassesA61K 51/04 (20060101)A61K 51/02 (20060101) A61K 51/10 (20060101) G01N 33/533 (20060101) G01N 33/551 (20060101) G01N 33/553 (20060101) AbstractA detectable molecule of the formulawhere A3 is A2 or a polymer, where A3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## --R1 -- is ##STR2## or a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where R2 is a C1 -C10 branched or unbranched alkyl; Za is chlorine, bromine or iodine; E is O, NH or an acyclic divalent sulfur atom; Detb is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR3## or the 4-hydroxy or acyloxy derivative thereof, where R3 is C1 -C4 alkyl or CH2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C1 -C10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A3. The detectable molecules are useful in in virto or in vivo assays or therapy.Other References
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