Patent 4687737 Issued on August 18, 1987. Estimated Expiration Date: July 2, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide424/204.1, Virus or component thereof424/205.1, Reassortant or deletion mutant virus435/235.1, VIRUS OR BACTERIOPHAGE, EXCEPT FOR VIRAL VECTOR OR BACTERIOPHAGE VECTOR; COMPOSITION THEREOF; PREPARATION OR PURIFICATION THEREOF; PRODUCTION OF VIRAL SUBUNITS; MEDIA FOR PROPAGATING435/239, Recovery or purification435/456, The polynucleotide is encapsidated within a virus or viral coat435/466, The polynucleotide is a shuttle vector or a transiently replicating hybrid vector435/69.3, Antigens435/69.4, Hormones and fragments thereof435/91.3, Polynucleotide contains only ribonucleotide monomers435/91.41, By insertion or addition of one or more nucleotides435/91.5, Acellular preparation of polynucleotide536/23.1DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)
A method of suppressing a nonsense codon in a gene in a mammalian cell by preparing an oligonucleotide primer having a mismatched anticodon region corresponding to the nonsense codon; preparing a DNA template for production of a tRNA molecule enabling the insertion of an amino acid when the nonsense codon is translated; forming a suppressor gene from said template and primer by site specific mutagenesis; and transforming the suppressor gene into the cell.
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