Process for amplifying nucleic acid sequences
Patent 4683202 Issued on July 28, 1987. Estimated Expiration Date: 
October 25, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
October 25, 2005. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.InventorAssigneeApplicationNo. 06/791308 filed on 10/25/1985US Classes:435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/317.1, MISCELLANEOUS (E.G., SUBCELLULAR PARTS OF MICRO-ORGANISMS, ETC.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.33PrimersExaminersPrimary: Martinell, JamesAttorney, Agent or FirmInternational ClassesC12Q 1/68 (20060101)C12Q 1/68 (20060101) C12N 15/10 (20060101) C12N 15/10 (20060101) B01L 7/00 (20060101) B01L 7/00 (20060101) C07K 14/795 (20060101) C07K 14/795 (20060101) C07K 14/805 (20060101) C07K 14/805 (20060101) AbstractThe present invention is directed to a process for amplifying any desired specific nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, and extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.Other References
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