Process for amplifying, detecting, and/or-cloning nucleic acid sequences

Patent 4683195 Issued on July 28, 1987. Estimated Expiration Date:

February 7, 2006. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Method for the direct analysis of sickle cell anemia Patent #: 4395486
Issued on: 07/26/1983
Inventor: Wilson , et al.

Inventors

Assignee

Cetus Corporation

Application

No. 06/828144 filed on 02/07/1986

US Classes:

435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/91.41, By insertion or addition of one or more nucleotides436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/508, Antinuclear (e.g., DNA, etc.)436/63, BIOLOGICAL CELLULAR MATERIAL TESTED436/94Saccharide (e.g., DNA, etc.)

Examiners

Primary: Martinell, James

Attorney, Agent or Firm

International Classes

C12Q 1/68 (20060101)
C12Q 1/68 (20060101)

Abstract

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.

Other References

Gorski et al, "Molecular organization of the HLA-SB Region of the Human Major Histocompatibility Complex and Evidence for Two SB Beta-chain Genes", Proc. Natl. Acad. Sci. USA 81: 3934 (1984)
Boss et al, "Cloning and Sequence Analysis of the Human Major Histocompatibility Complex Gene DC-3beta", Proc. Nat. Acad. Sci. USA 81: 5199 (1984)
Okada et al, "Gene Organization of DC and DX Subregions of the Human Major Histocompatibility Complex", Proc. Natl. Acad. Sci. USA 82: 3410 (1985)
Salser, "Cloning cDNA sequences: A general Technique for Propagating Eukaryotic Gene Sequences in Bacterial Cells", in Genetic Engineering, 1978, Charrabarty (ed.), CRC Press, Inc. Boca Raton, Fla., pp. 53-81
Gaubatz et al, "Strategies for Constructing Complementary DNA for Cloning", J. Theor. Biol. 95: 679 (1982)
Rossi et al., J. Biol. Chem., 257, 9226-9229 (1982)