Patent 4681852 Issued on July 21, 1987. Estimated Expiration Date: July 21, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
A method for producing phenylalanine comprising culturing novel strains of the bacteria Escherichia coli. The novel strains of E. coli are those in which inhibition of the enzyme DAHP synthease by phenylalanine, tyrosine or tryptophan is removed and inhibition of the enzyme chorismate mutase P-prephenate dehydratase by phenylalanine is removed and in which enhanced levels of production of the enzymes DAHP synthease, chorismate mutase P-prephenate dehydratase and shikimate kinase are achieved. The organism is preferably also modified to limit the production of alternate end products from chorismate.
Tribe et al, 1979, "Hyperproduction of Tryptophan by E. coli, Genetic Manipulation of the Pathways Leading to Tryptophan Formation", Applied & Env. Micro, v 38(2), pp. 181-190
Faculty Handbook, Univ. New South Wales, pp. 204-205, 1985
Lehninger, A., Biochemistry, Worth Publishers, Inc., New York, pp. 708-713, (1970)
Zurawski, G. et al, Proc. Natl. Acad. Sci., vol. 75(10), pp. 4271-4275, (1978), "Nucleotide Sequence of the Leader Region of the Phenylalanine Operon of Escherichia coli"
Camakaris H. et al., Jour. of Bacteriol., vol. 150(1), pp. 70-75, (1982), "Autoregulation of the tyr R Gene"
Im, S. W. K. et al, Jour. of Bacteriol., vol. 108(1), pp. 400-409, (1971), "Phenylalanine and Tyrosine Biosynthesis in Escherichia coli K-12: Mutants Derepressed for 3-Deoxy-D-Arabinoheptulosonic Acid-7-Phosphate Synthetose (phe), 3-Deoxy-D-Arabinoheptulsonic Acid-7-Phosphate Synthetose (tyr), Chorismate Mutase T-Prephanate Dehydrogenase, and Transaminase A"
Herrmann, K. M. et al, editors, Amino Acids: Biosynthesis and Genetic Regulation, Addison-Wesley Pub. Co., Mass., pp. 323-338, (1983)
Walker, R., The Molecular Biology of Enzyme Synthesis, John Wiley, and Sons, New York, pp. 186-187, (1983)
Chemical Abstracts, vol. 98, p. 145, 1983, abstract no. 12330u: Plumridge, J. A. et al, "Escherichia coli Phenylalanyl-tRNA Synthetose Operon: Characterization of Mutations Isolated on Multicopy Plasmids"
Chemical Abstracts, vol. 98, p. 116, 1983, abstract no. 1197e: Hennecke, H. et al, "A Novel Cloning Vector for the Direct Selection of Recombinant DNA in E. coli"
Jacobs, N. J. et al, "Characterization of the Late Steps of Microbial Heme Synthesis: Conversion of Coproporphyrinogen to Protophyrin", J. Bactiorolog., vol. 107:1, pp. 203-209, (1971)
Im, S. W. K. et al., "Phenylalanine Biosynthesis in Escherichia Coli K-12: Mutants Derpressed for Chorismate Mutase P-Prephenate Dehydratase", J. Bactiorolog., vol. 106:3, 784-790, (1971)
Brown, K. D. et al, "Repression of Aromatic Amino Acid Biosynthesis in Escherichia Coli K-12", J. Bacteriolog., vol. 108:1, pp. 386-399, (1971)
Im, S. W. K., et al., "Phenylalanine and Tyrosine Biosynthesis in Escherichia coli K-12: Mutants Depressed for 3-eoxy-D-Arabinopehtulosonic Acid 7-Phosphate Synthetase (phe), 3-Deoxy-D-Arabinoheptulosonic Acid 7-Phosphate Synthetase (tyr), Chorismate Mutase T-Prephenate Dehydrogenase and Transmitase A", J. Bacteriolog., 108:1, 400-409, (1971)
Ely, B. et al, "Aromatic Amino Acid Biosynthesis: Regulation of Shikimate Kinase in Escherichia coli K-12", J. Bacteriolog., 138:3, 933-943, (1949)
Bachman, B. J. et al, "Linkage Map of Escherichia coli K-12, Edition 6", Microbiological Reviews, vol. 44:1, pp. 1-56
Kleckner, N. et al, "Genetic Engineering In Vivo Using Translocatable Drug-Resistance Elements", J. Mol. Biol., vol. 116, pp. 125-159, (1977)