Integral element for analysis of liquids
Reagent and method for the analytic determination of hydrogen peroxide
Determination of hydrogen peroxide
Method and reagent for the quantitative determination of hydrogen peroxide and precursors thereof
Azo dyes derived from 3-amino-2,1-benzisothiazoles and aromatic amine couplers containing sulfo groups, or salts thereof
Azo dyes derived from 5-membered heterocyclic amines and aromatic amine couplers containing sulfo groups, or salts thereof
System for the determination of glucose in fluids
ApplicationNo. 06/678931 filed on 12/06/1984
US Classes:435/10, Involving uric acid435/14, Involving glucose or galactose435/28, Involving peroxidase435/805, TEST PAPERS435/810, PACKAGED DEVICE OR KIT436/66, HEMOGLOBIN, MYOGLOBIN, OR OCCULT BLOOD546/171, Nitrogen, other than as nitro or nitroso, attached directly to the carbocyclic ring of the quinoline ring system by nonionic bonding546/172, Acyclic sulfur bonded directly to oxygen and directly or indirectly to the quinoline ring system by nonionic bonding546/23, Polycyclo ring system having the six-membered hetero ring as one of the cyclos548/491, Chalcogen or nitrogen attached indirectly to the five-membered hetero ring by acyclic nonionic bonding987/95Quinoline or hydrogenated quinoline ring (9/60)
ExaminersPrimary: Warden, Robert J.
Assistant: Deck, Randall E.
Attorney, Agent or Firm
International ClassesC12Q 1/28 (20060101)
C12Q 1/54 (20060101)
C12Q 1/62 (20060101)
C07F 9/00 (20060101)
C07F 9/60 (20060101)
G01N 33/52 (20060101)
AbstractParticular heterocyclic compounds are useful as color-forming couplers in analytical compositions, elements and methods. These compounds are represented by the structure: ##STR1## wherein R is a solubilizing group, R1, R2, R3 and R4 are independently hydrogen, alkyl, alkoxy, aryl, aryloxy, a heterocyclic group, halo, amino or R, and Z represents the carbon atoms necessary to complete the nucleus of a 5- or 6-membered fused ring. The color-forming coupler is combined with an oxidizable color developing compound, e.g. 4-aminoantipyrine to provide a chromogenic composition. This composition can be used in solution or dry assays of biological fluids to produce a color dye in the presence of hydrogen peroxide or other analytes, e.g. glucose or uric acid, which react to produce hydrogen peroxide.