U.S. patents available from 1976 to present.
U.S. patent applications available from 2005 to present.

Process for regenerating corn

Patent 4665030 Issued on May 12, 1987. Estimated Expiration Date: Icon_subject September 7, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventor

Assignee

Application

No. 06/648389 filed on 09/07/1984

US Classes:

435/424Involving corn cell or tissue

Examiners

Primary: Wiseman, Thomas G.
Assistant: Fox, David T.

Attorney, Agent or Firm

International Class

A01H 4/00 (20060101)

Abstract

The present invention relates to the regeneration of corn. The process comprises the steps of:(a) culturing tissue obtained from a corn plant on a first medium which comprises mineral salts, vitamins sucrose and a hormone in an amount sufficient to ensure callus formation;(b) subculturing the calli on a second medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus maintenance;(c) subculturing the calli on a third medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot and root formation; and(d) optionally subculturing said shoots on a fourth medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficent to ensure plantlet maturation including additional root formation, whereby plants are obtained.

Other References

  • Vasil (Editor) 1984, Cell Culture and Somatic Cell Genetics of Plants, Academic Press, pp. 19-21 and 26
  • Conger (Editor) 1981, Cloning Agricultural Plants Via in Vitro Techniques, CRC Press, pp. 13, 175
  • Dudits et al, 1975, "Study of Callus Growth and Organ Formation in Wheat (Triticum Aestivum)", Can J. Bot., vol. 53, pp. 957-963
  • Earle et al, 1982, Variability in Plants Regenerated from Tissue Culture, Praeger, p. 6
  • Evans et al, 1983, Handbook of Plant Cell Culture, vol. 1, Macmillan Publ. Co., pp. 101-102
  • Green, C. E., et al., Crop Science 15, 417 (1975)
  • Freeling, M., et al., Maydica 21, 97 (1976)
  • Lu, C., et al., Theor. Appl. Genet. 66, 285 (1983)
  • Edallo, S., et al., Maydica 26, 39 (1981)
  • Lu, C., et al., Theor. Appl. Genet. 62, 109 (1982)
  • Hibberd, K. A., et al., Proc. Natl. Acad. Sci. USA 79, 559 (1982)
  • Gengenbach, B. G. et al., Proc. Natl. Acad. Sci. USA 74, 5113 (1977)
  • Green, C. E., et al., Crop Science 14, 54 (1974)
  • Irvine, J. E., et al., Plant Cell Tissue Organ Culture 2, 141 (1983)
  • Hanning, G. E., et al., Theor. Appl. Genet. 63, 155 (1982)
  • Conger, B. V., et al., Science 221, 850 (1983)
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