Biologically active composition and the use thereof
Method for distinguishing subpopulations in a population of morphologically indistinguishable cells
Method for detecting and quantifying antigens
Method of producing an R-type bacteriocin and its use in the detection of specific microorganisms
Production of urokinase
Process for rosetting human B lymphocytes with Rhesus monkey erythrocyte
ApplicationNo. 06/443191 filed on 11/23/1982
US Classes:435/7.21, Animal cell435/177, Enzyme or microbial cell is immobilized on or in an organic carrier435/178, Carrier is carbohydrate435/182, Enzyme or microbial cell is entrapped within the carrier (e.g., gel, hollow fibre)435/7.23, Tumor cell or cancer cell435/7.24, Leukocyte (e.g., lymphocyte, granulocyte, monocyte, etc.)435/7.25, Erythrocyte435/803, PHYSICAL RECOVERY METHODS (E.G., CHROMATOGRAPHY, GRINDING)435/966, INVOLVING AN ENZYME SYSTEM WITH HIGH TURNOVER RATE OR COMPLEMENT MAGNIFIED ASSAY (E.G., MULTI-ENZYME SYSTEMS, ETC.)436/512, INVOLVING ANTIBODY FRAGMENTS436/520, Red blood cell436/521, Fixation or stabilization of red blood cells436/522, Lysis of red blood cell membrane436/535, Antigen or antibody entrapped within the carrier (e.g., gel, hollow fiber, etc.)436/536, INVOLVING IMMUNE COMPLEX FORMED IN LIQUID PHASE436/539, Involving precipitating reagent436/821, INVOLVING COMPLEMENT FACTORS OR COMPLEMENT SYSTEMS436/824IMMUNOLOGICAL SEPARATION TECHNIQUES
ExaminersPrimary: Wiseman, Thomas G.
Assistant: Weimar, Elizabeth C.
Attorney, Agent or Firm
International ClassesC12Q 1/24 (20060101)
C12N 5/06 (20060101)
C12N 5/16 (20060101)
G01N 33/554 (20060101)
G01N 33/563 (20060101)
AbstractThe invention relates to techniques for isolating from a mixed population of cells disired living cells either producing and releasing a particular product or having a characteristic molecule on their surface. The isolation techniques depend upon the localized interaction between the product (or molecule) and other agents added to the system such that distinguishable conditions can be caused to occur (or not occur) only in the immediate vicinity of desired cells which produced and released the product or which contain the molecule on their surface.
Field of SearchEnzyme or microbial cell is immobilized on or in an organic carrier
Carrier is carbohydrate
Enzyme or microbial cell is entrapped within the carrier (e.g., gel, hollow fibre)
Separation of micro-organism from culture media
PHYSICAL RECOVERY METHODS (E.G., CHROMATOGRAPHY, GRINDING)
ENZYME SEPARATION OR PURIFICATION