Hybridization method of detecting nucleic acid sequences with probe containing thionucleotide

Patent 4647529 Issued on March 3, 1987. Estimated Expiration Date:

June 1, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

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Inventors

Application

No. 06/616286 filed on 06/01/1984

US Classes:

435/6, Involving nucleic acid435/78, Oxygen atom of the saccharide radical is directly bonded to a condensed ring system having three or more carboxyclic rings (e.g., dauomycin, adriamycin, etc.)436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.5, Nucleic acid expression inhibitors536/25.32, Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.)536/25.4, Separation or purification of polynucleotides or oligonucleotides536/25.6, Nucleic acids which include two or three nucleotide units536/26.5Plural N-glycosidic moieties bonded to the same phosphorus ester group

Examiners

Primary: Marantz, Sidney
Assistant: Krawczewicz, L.

Attorney, Agent or Firm

Klarquist, Sparkman, Campbell, Leigh & Whinston

International Classes

C12Q 1/68 (20060101)
C07H 21/00 (20060101)

Abstract

Improved hybridization probe compositions and method for detecting nucleic acids are provided. A hybridization probe constructed to contain thionucleotides is hybridized to a nucleotide sequence of interest in the absence of formamide, DTT and dextran sulfate. If a cold (32 S) thionucleotide is incorporated into the probe molecule, the probe is provided with a detectable label, radioactive or otherwise.

Other References

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