Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials

Patent 4634665 Issued on January 6, 1987. Estimated Expiration Date:

January 6, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Inventors

Application

No. 06/522408 filed on 08/11/1983

US Classes:

435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/465, Involving co-transfection435/69.3, Antigens435/69.4, Hormones and fragments thereof435/69.5, Lymphokines or monokines435/69.51, Interferons435/69.52, Interleukins435/69.6, Blood proteins435/811, INTERFERON435/948, Using viruses or cell lines536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/23.51, Hormone536/23.52Interferon

Examiners

Primary: Kepplinger, Esther M.

Attorney, Agent or Firm

White; John P.

International Classes

C12P 21/02 (20060101)
C12N 15/85 (20060101)

Abstract

The present invention relates to processes for inserting DNA into eucaryotic cells, particularly DNA which includes a gene or genes coding for desired proteinaceous materials for which no selective criteria exist. The insertion of such DNA molecules is accomplished by cotransforming eucaryotic cells with such DNA together with a second DNA which corresponds to a gene coding for a selectable marker.This invention also concerns processes for producing proteinaceous materials such as insulin, interferon protein, growth hormone and the like which involve cotransforming eucaryotic cells with DNA which codes for these proteinaceous materials, growing the contransformed cells for production of the proteinaceous material and recovering the proteinaceous material so produced.The invention further relates to processes for inserting into eucaryotic cells a multiplicity of DNA molecules which includes genes coding for desired proteinaceous materials. The insertion of multiple copies of desired genes is accomplished by cotransformation with the desired genes and with amplifiable genes for a dominant selectable marker in the presence of successively higher amounts of an inhibitor. Alternatively, the insertion of multiple copies of desired genes is accomplished by transformation using DNA molecules formed by ligating a DNA molecule including the desired gene to a DNA molecule which includes an amplifiable gene coding for a dominant selectable phenotype such as a gene associated with resistance to a drug in the presence of successively higher amounts of an agent such as a drug against which the gene confers resistance so that only those eucaryotic cells into which multiple copies of the amplifiable gene have been inserted survive. Eucaryotic cells into which multiple copies of the amplifiable gene have been inserted additionally include multiple copies of the desired gene and may be used to produce multiple copies of proteinaceous molecules. In this way otherwise rare proteinaceous materials may be obtained in higher concentrations than are obtainable using conventional techniques.

Other References

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