Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes
April 27, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 2126852 3617185 Differential counting of leukocytes and other cells Method for the determination of leukemic cells Metachromatic dye sorption means for differential determination of leukocytes Method for discriminating between unstained and absorbing dye stained cells Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes Individual leukocyte determination by means of differential metachromatic dye sorption Patent #: 4581223 InventorApplicationNo. 06/604869 filed on 04/27/1984US Classes:435/34, Determining presence or kind of micro-organism; use of selective media435/39, Quantitative determination436/63BIOLOGICAL CELLULAR MATERIAL TESTEDExaminersPrimary: Nucker, Christine M.Assistant: Foulke, Cynthia Lee Attorney, Agent or FirmInternational ClassesG01N 33/50 (20060101)G01N 1/30 (20060101) AbstractDifferentiation, identification and enumeration of sub-populations of lymphocytes including B-cells and T-cells can now be further discriminated into their sub-populations by means of basic orange 21 as a supravital stain to provide distinctive morphologic differences between B-cells, T-suppressor cells, T-helper cells and natural killer (NK) cells without resorting to the use of individual monoclonal antibodies for differentiation of each of the above.The differentiation, identification and enumeration of the foregoing cells can be accomplished by means of both white light absorbance and fluorescence measurements, singly or in sequential combinations.Other References
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