Abstract

A novel and improved method to detect and/or measure indirectly at least one gas, particularly dissolved gases, of an animal employing nuclear magnetic resonance techniques (NMR). The method examines an NMR spectrum to determine chemical shift relaxation times or spin-spin couplings for an element of an animal influenced by at least one gas of the animal. Uniquely, the gas detected according to the principles of this invention may be insensitive to present NMR techniques. Further, the present invention discloses a novel method to determine and monitor gaseous physiological states of an animal. Because of the unique and advantageous non-invasive, non-destructive and non-ionizing properties, the present invention may be employed in an animal continuously and in vivo. Still further, the present invention provides for the imaging of at least one-, two-, or three-dimensional projections reconstructed from a chemical shift, relaxation times, or spin-spin couplings for an element of an animal influenced by at least one gas in the animal. Novel compounds directed to providing radio-frequency reference signals in which chemical shifts may be determined therefrom are also disclosed.

Other References

• Mansfield, P. et al., "NMR Imaging in Biomedicine", Academic Press, 1982, New York, N.Y., p. 230
• Gore, J. C. et al., "NMR Imaging at Hammersmith Hospital", SPIE vol. 273, Application of Optical Instr. in Medicine IX, 1981
• Harrison's Textbook of Medicine, p. I-124, McGraw Hill (10th Ed.) 1983
• Holland, G. N. et al.: ¹⁹ F. Magnetic Resonance Imaging, J. Magnetic Resonance, 28:133-136 (1977)
• Thomas, S. R. et al.: Nuclear Magnetic Resonance Imaging Techniques as Developed Modestly within a University Medical Center Environment: What Can the Small System Contribute at this Point?, Magnetic Resonance Imaging, 1(1):11-21 (1981)
• Delpuech, J. J., Hamza, M. A., and Serratrice, G.: Determination of Oxygen by a Nuclear Magnetic Resonance Method, J. Magnetic Resonance, 36:173-179 (1979)
• Hamza, M. A. et al.: Fluorocarbons as Oxygen Carriers, II, An NMR Study of Partially or Totally Fluorinated Alkanes and Alkenes, J. Magnetic Resonance, 42:227-241 (1981)
• Hall, L. D. and Sukuman, S.: Chemical Microscopy Using a High-Resolution NMR Spectrometer, A Combination of Tomography/Spectroscopy Using Either ¹ H or ¹³ C, 50:161-164 (1982)
• Lauterbur et al.: Zeugmatographic High Resolution Nuclear Magnetic Resonance Spectroscopy Images of Chemical Inhomogeneity Within Macroscopic Objects, J. American Chemical Society, 97(23):6866-6868, Nov. 12, 1975
• Hamza, M. A. et al.: Solute-Solvent Interactions in Perfluorocarbon Solutions of Oxygen, An NMR Study, J. American Chemical Society, 103(13):3733-3738 (1981)