Detection of microbial nucleic acids by a one-step sandwich hybridization test
Patent 4563419 Issued on January 7, 1986. Estimated Expiration Date: 
December 29, 2003. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
December 29, 2003. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesImmobilized immunoadsorbent Nucleic acid and protein binding paper Transfer and detection of nucleic acids Specific DNA probes in diagnostic microbiology Autonomously replicating DNA containing inserted DNA sequences Patent #: 4359535 InventorsAssigneeApplicationNo. 06/566532 filed on 12/29/1983US Classes:435/6, Involving nucleic acid435/188, Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP435/5, Involving virus or bacteriophage435/810, PACKAGED DEVICE OR KIT436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/504, Radioactive label436/804, RADIOISOTOPE (E.G., RADIOIMMUNOASSAY, ETC.)436/808, Automated or kit436/811, TEST FOR NAMED DISEASE, BODY CONDITION OR ORGAN FUNCTION436/823, IMMUNOGENIC CARRIER OR CARRIER PER SE536/23.7, Encodes a microbial polypeptide536/24.32Probes for detection of microbial nucleotide sequencesExaminersPrimary: Wiseman, Thomas G.Assistant: Moskowitz, M. Attorney, Agent or FirmInternational ClassesC12Q 1/68 (20060101)C12Q 1/70 (20060101) C12Q 1/04 (20060101) Foreign Application Priority Data1981-10-16 FIAbstractThis invention relates to a kit for the detection of microbial nucleic acids and a method for identifying the nucleic acids using a one-step sandwich hybridization technique. The technique requires two complementary nucleic acid reagents for each microbe or group of microbes to be identified.Other References
Field of SearchBIOSPECIFIC LIGAND BINDING ASSAYUtilizing isolate of tissue or organ as binding agent Radioactive label FLUORESCENT DYES (E.G., RHODAMINE, ETC.) RADIOISOTOPE (E.G., RADIOIMMUNOASSAY, ETC.) Automated or kit Multifield plates or multicontainer arrays Tube, bottle, or dipstick TEST FOR NAMED DISEASE, BODY CONDITION OR ORGAN FUNCTION TEST FOR NAMED COMPOUND OR CLASS OF COMPOUNDS IMMUNOGENIC CARRIER OR CARRIER PER SE BIOLOGICAL CELLULAR MATERIAL TESTED |
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