DNA for directing transcription and expression of structural genes
Patent 4559302 Issued on December 17, 1985. Estimated Expiration Date: 
December 17, 2002. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
December 17, 2002. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesProcess for producing biologically functional molecular chimeras Plasmid vectors, production anduse thereof Patent #: 4349629 InventorAssigneeApplicationNo. 06/438070 filed on 11/01/1982US Classes:435/252.33, Escherichia (e.g., E. coli, etc.)435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/252.35, Streptomyces435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/69.3, Antigens435/69.4, Hormones and fragments thereof435/69.51, Interferons435/849, Escherichia coli435/886, Streptomyces536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/23.51, Hormone536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)536/24.2Non-coding sequences having no known regulatory function which are adaptors or linkers for vector or gene contructionExaminersPrimary: Wiseman, Thomas G.Assistant: Teskin, Robin L. Attorney, Agent or FirmInternational ClassC12N 15/70 (20060101)AbstractThe present invention relates to a novel transcriptional and translational activating sequence. The novel activating sequence can be either chemically synthesized or isolated on a 0.17 kb PstI-SacI restriction fragment from plasmid pKC203, a plasmid of E. coli JR225 (ATCC 31912). The activating sequence directs expression of the aminoglycoside acetyltransferase aac(3)IV and hygromycin phosphotransferase aph(4) genes present on plasmid pKC203. A series of expression vectors have been constructed in which the activating sequence directs the expression of beta-galactosidase or hygromycin phosphotransferase. These vectors can be readily modified and have been designed to facilitate the subsequent cloning and expression of any gene of research or commercial interest. The expression and cloning vectors have been transformed into E. coli and other host cells in which the activating sequence functions.Other References
|
| ||||||||||||||
