Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
Patent 4535060 Issued on August 13, 1985. Estimated Expiration Date: 
January 5, 2003. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
January 5, 2003. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesProduction of xylose (dextrose) isomerase enzyme preparations Process for producing biologically functional molecular chimeras Method for preparing strains which produce aminoacids Patent #: 4278765 InventorAssigneeApplicationNo. 06/455634 filed on 01/05/1983US Classes:435/252.33, Escherichia (e.g., E. coli, etc.)435/193, Transferase other than ribonuclease (2.)435/232, Lyase (4. )435/252.1, Bacteria or actinomycetales; media therefor435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/317.1, MISCELLANEOUS (E.G., SUBCELLULAR PARTS OF MICRO-ORGANISMS, ETC.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/91.41, By insertion or addition of one or more nucleotides435/91.53, Involving a hydrolase (3.)536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/23.2Encodes an enzymeExaminersPrimary: Wiseman, Thomas G.Assistant: Huleatt, Jayme A. Attorney, Agent or FirmInternational ClassesC12N 15/82 (20060101)C12N 9/10 (20060101) AbstractEnhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3-phosphoshikimate synthetase (EC: 2.5.1.19) (ES-3-P synthetase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme.The E. coli strain C600(pPMG1) has been deposited at the A.T.C.C. on Dec. 14, 1982 and been given A.T.C.C. accession no. 39256.Other References
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