Multiple reactor system and method for polynucleotide synthesis
Patent 4517338 Issued on May 14, 1985. Estimated Expiration Date: 
July 13, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
July 13, 2004. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesChemical synthesis apparatus for preparation of polynucleotides Solid-phase synthesis of polynucleotides Process for preparing polynucleotides Patent #: 4458066 InventorsAssigneeApplicationNo. 06/630781 filed on 07/13/1984US Classes:536/25.3, Synthesis of polynucleotides or oligonucleotides422/116, Sensed condition is operating time and control is operation sequencer422/131, Organic polymerization435/317.1, MISCELLANEOUS (E.G., SUBCELLULAR PARTS OF MICRO-ORGANISMS, ETC.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/820, SUBCELLULAR PARTS OF MICRO-ORGANISMS525/54.1, Containing chemically combined protein or biologically active polypeptide525/54.23, Previously formed solid polymer is derived from ethylenically unsaturated reactants only536/25.34Trivalent phosphorus compound utilizedExaminersPrimary: Kight, JohnAssistant: Nutter, Nathan M. Attorney, Agent or FirmInternational ClassesB01J 19/00 (20060101)C07H 21/00 (20060101) AbstractA reactor system and method for synthesizing or degrading polynucleotides and other linear polymers includes a tubular reactor connected to a reagent manifold. The polynucleotide is immobilized on a loosely packed solid-phase support material in the tubular reactor, and reagents are sequentially introduced into the tubular reactor. After each reagent is introduced, the tubular reactor is isolated from the reagent manifold and the reagent agitated by alternately pressurizing the opposite ends of the tubular reactor. The method provides rapid and efficient synthesis of polynucleotides. By connecting two or more tubular reactors to the reagent manifold, a plurality of polynucleotides having different sequences may be synthesized simultaneously.Other References
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