Simultaneous assay for T and B lymphocyte populations and subpopulations
June 18, 2002. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3088875 3853987 Cell specific, variable density, polymer microspheres Antibody coated bacteria Reagent combination for solid phase immunofluorescent assay Method and apparatus for automated identification and enumeration of specified blood cell subclasses Complement-fixing monoclonal antibody to human T cells, and methods of preparing same Complement-fixing monoclonal antibody to human suppressor T cells and methods of preparing same Monoclonal antibody to human T cells, and methods for preparing same Monoclonal antibody to human cytotoxic and suppressor T cells and methods of preparing same InventorsAssigneeApplicationNo. 06/389838 filed on 06/18/1982US Classes:436/513, INVOLVING IGA, IGD, IGE, OR IGM436/519, Carrier is a biological cell or cell fragment436/523, Carrier is particulate and the particles are of intentionally different sizes or impregnated differently with the immunochemicals436/531, Carrier is synthetic resin436/533, Carrier is water suspendible particles (e.g., latex, etc.)436/534, Carrier is water suspendible particles436/548Monoclonal antibodyExaminersPrimary: Kepplinger, Esther M.Attorney, Agent or FirmInternational ClassesA61K 35/26 (20060101)G01N 33/569 (20060101) AbstractAn assay for simultaneously determining the ratio of B cells and T cells to the total cell population and subpopulations thereof present in a lymphocyte population utilizes excess amounts of B cell binding protein and T cell binding protein bound to solid phase particles. By exposing the particles to the lymphocyte population, rosettes are formed which may be visually distinguished and counted under a microscope, yielding the proportions of B cells and T cells, including any subpopulations for which a specific label was provided. Macrophages may be identified by their ability to ingest the particles.Other References
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