ApplicationNo. 06/412648 filed on 08/30/1982
US Classes:422/52, Chemiluminescent250/461.2, Biological cell identification422/73, With coagulometer for agglutination, clotting, or prothrombin, or for particle (e.g., cell, etc.) counting, or volume or characteristics determination422/82.08, Fluorescence422/82.09, Absorbance or transmittance436/172, With fluorescence or luminescence436/63BIOLOGICAL CELLULAR MATERIAL TESTED
ExaminersPrimary: Turk, Arnold
Attorney, Agent or Firm
International ClassesG01N 33/50 (20060101)
G01N 33/569 (20060101)
G01N 15/14 (20060101)
AbstractA method of distinguishing multiple subpopulations of cells from a single sample of cells of a variety of types comprises labeling particles with two or more marking agents. These particles are marked in a plurality of different pre-selected ratios of the agents ranging between zero percent and one hundred percent of each agent. Each such agent has distinguishing, quantifiable marking characteristics. The differently labeled particles are mixed with cells suspected of having specific receptors for the differently labeled particles. Each cell is analyzed to determine the ratio of any two identifiable marking characteristics associated with each cell so that it can be classified in a subpopulation category if its ratio of marking characteristics is related to one of the pre-selected ratios of marking agents.An apparatus for carrying out the above-described method is also within the purview of the present invention.