Electrophoresis using alternating transverse electric fields
Patent 4473452 Issued on September 25, 1984. Estimated Expiration Date: 
November 18, 2002. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
November 18, 2002. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod of electrophoretic purification of enzymes and peptides by means of an adjustable, specialized, geometrically located electrode system Patent #: 4148703 InventorsApplicationNo. 06/442580 filed on 11/18/1982US Classes:204/458, Plural rapid changes in direction of electric field (at least 1,000 times total and at more than 1/sec) (e.g., pulsed field, etc.)204/609Plural rapid changes in direction of electric field (at least 1,000 times total and at more than 1/sec) (e.g., pulsed field, etc.)ExaminersPrimary: Niebling, John F.Assistant: Boggs, B. J. Jr. Attorney, Agent or FirmInternational ClassG01N 27/447 (20060101)AbstractDisclosed are an apparatus for and a method of electrophoretically separating particles by electric fields which are transverse to each other, which alternate between respective high and low intensities out of phase with each other at a frequency related to the mass of the particles and which move the particles in an overall direction transverse to the respective directions of the fields. For separating large macromolecules, at least one of the fields preferably has an intensity gradient in a direction transverse to its own. The new arrangement makes it possible to: (1) separate particles (molecules) larger in size than those able to be separated with previously known techniques, (2) carry out separation at higher speed and at better resolution than is possible with previously known techniques, and (3) concurrently separate particles which differ greatly in mass (molecular weight). |
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