Vitamin B12 and labelled derivatives for such assay
Patent 4465775 Issued on August 14, 1984. Estimated Expiration Date: 
May 24, 2002. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
May 24, 2002. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesCyanocobalamin derivatives Folic acid derivatives and use in radio-assay 4091087 Vitamin B12 derivative suitable for radiolabeling Radiolabeled derivatives of folic acid Folic acid derivatives Folic acid derivatives Patent #: 4336185 InventorAssigneeApplicationNo. 06/381267 filed on 05/24/1982US Classes:436/503, Utilizing isolate of tissue or organ as binding agent435/14, Involving glucose or galactose435/21, Involving phosphatase435/25, Involving oxidoreductase435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP436/504, Radioactive label436/505, B12 or folate436/545, Radioactive label436/546, Fluorescent label536/26.4, Cobalamin nucleotides (e.g., vitamin B-12, etc.)536/26.41, Processes of preparing or labelling536/26.44Cobalamin analogs (i.e., compounds wherein the benzimidazole ring system has been replaced by another organic ring structure, or compounds wherein cobalt has been removed or replaced by another metal, or is substituted by a group other than -OH or -CN)ExaminersPrimary: Padgett, Benjamin R.Assistant: Moskowitz, M. Attorney, Agent or FirmInternational ClassesG01N 33/82 (20060101)C07H 23/00 (20060101) Foreign Application Priority Data1981-06-22 GBAbstractVitamin B12 in liquid samples, such as human serum or human plasma, is assayed by a competitive binding technique using intrinsic factor and certain labelled vitamin B12 derivatives. The labelled derivatives are formed from the (d)-monocarboxylic acid isomer of vitamin B12, which isomer is free from other monocarboxylic acid isomers (and derivatives thereof) of vitamin B12, by binding to the (d)-isomer, via the carboxylic group, a compound which is itself a label (e.g. an enzyme) or which comprises a label (e.g. a fluorophore), or to which a label is attached (e.g. a histidine ester to which 125 I is attached). The labelled derivatives of the (d)-monocarboxylic acid of vitamin B12, free from other isomeric monocarboxylic acids and derivatives, are novel and constitute one aspect of the invention.Other References
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