Partially purified osteogenic factor and process for preparing same from demineralized bone
Patent 4434094 Issued on February 28, 1984. Estimated Expiration Date: 
April 12, 2003. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
April 12, 2003. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3539549 Bone morphogenetic protein process Patent #: 4294753 InventorsAssigneeApplicationNo. 06/484286 filed on 04/12/1983US Classes:530/416, Ion exchange424/549, Skeleton (e.g., bone, teeth, etc.)530/840, Bones; tendons; teeth; cartilage623/919BoneExaminersPrimary: Schain, Howard E.Attorney, Agent or FirmInternational ClassesA61L 27/00 (20060101)A61L 27/22 (20060101) C07K 14/435 (20060101) C07K 14/51 (20060101) A61K 38/00 (20060101) AbstractA process for partially purifying an osteogenic factor from demineralized bone particles in which nonfibrous proteins are extracted from the demineralized bone with a dissociative extractant for nonfibrous proteins, such as urea or guanidine hydrochloride, the extracted nonfibrous proteins are fractionated by anion exchange chromatography using DEAE cellulose at pH 7, the fraction not adsorbed by the DEAE cellulose is further fractionated by cation exchange chromatography using CM cellulose at pH 4.8, the fraction adsorbed by the CM cellulose is eluted therefrom and the partially purified osteogenic factor is recovered from the eluate as a ࣘ30,000 dalton protein isolate.Other References
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